Fluorescent poly[N-(2-hydroxypropyl) methacrylamide] nanogel by dispersion polymerization as a contrast agent for live-cell imaging

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F23%3A00574199" target="_blank" >RIV/61389013:_____/23:00574199 - isvavai.cz</a>

Alternative codes found

RIV/44555601:13440/23:43897726

Result on the web

<a href="https://onlinelibrary.wiley.com/doi/10.1002/app.54331" target="_blank" >https://onlinelibrary.wiley.com/doi/10.1002/app.54331</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/app.54331" target="_blank" >10.1002/app.54331</a>

Result language

angličtina

Original language name

Fluorescent poly[N-(2-hydroxypropyl) methacrylamide] nanogel by dispersion polymerization as a contrast agent for live-cell imaging

Original language description

Here, we report a novel dispersion polymerization for the preparation of cross-linked poly[N-(2-hydroxypropyl) methacrylamide] (PHPMA)-based nanogels in water/2-methoxyethanol mixture (H2O/MetCel), initiated with potassium persulfate (KPS), and stabilized with poly(vinyl alcohol) 25/140 (PVA) and sodium dodecyl sulfate (SDS). Obtained nanogels were characterized using transmission (TEM) and cryogenic transmission electron microscopy (cryo-TEM), dynamic light scattering (DLS), asymmetric flow field-flow fractionation (A4F), nuclear magnetic resonance spectroscopy (NMR), and Raman spectroscopy methods in terms of size, particle size distribution, morphology, and structure. N-(2-hydroxypropyl) methacrylamide (HPMA) was copolymerized with 20 wt% ethylene dimethacrylate (EDMA) resulting in 138 nm poly[N-(2-hydroxypropyl) methacrylamide-co-ethylene dimethacrylate] (PHPMA-EDMA) nanogel dispersion with irregular shape and core-shell type structure. Next, we copolymerized HPMA with 20 wt% EDMA and 10 wt% propargyl methacrylate (PMA) to incorporate reactive functionality into the final core-shell type 120 nm poly[N-(2-hydroxypropyl) methacrylamide-co-ethylene dimethacrylate-co-propargyl methacrylate] (PHPMA-EDMA-PMA) nanogel dispersion. Then, the biocompatibility of PHPMA-EDMA-PMA nanogel was proved using rat mesenchymal stem cells (rMSC), and human foreskin fibroblasts (BJ). PHPMA-EDMA-PMA nanogel was fluorescently labeled with sulfo-cyanine3 azide resulting in 131 nm nanogel. We performed in vitro uptake studies with fluorescently labeled PHPMA-EDMA-PMA nanogel using rMSC showing that the fluorescently labeled PHPMA-EDMA-PMA nanogel was well-distributed in the cytosol and taken up into lysosomes.n

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10404 - Polymer science

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Applied Polymer Science

ISSN

0021-8995

e-ISSN

1097-4628

Volume of the periodical

140

Issue of the periodical within the volume

34

Country of publishing house

US - UNITED STATES

Number of pages

14

Pages from-to

e54331

UT code for WoS article

001024203900001

EID of the result in the Scopus database

2-s2.0-85164310907