Hybrid macromolecular constructs as a platform for spectral nanoprobes for advanced cellular barcoding in flow cytometry
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F24%3A00583051" target="_blank" >RIV/61389013:_____/24:00583051 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11130/24:10467561 RIV/00064203:_____/24:10467561
Result on the web
<a href="https://onlinelibrary.wiley.com/doi/10.1002/mabi.202300306" target="_blank" >https://onlinelibrary.wiley.com/doi/10.1002/mabi.202300306</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/mabi.202300306" target="_blank" >10.1002/mabi.202300306</a>
Alternative languages
Result language
angličtina
Original language name
Hybrid macromolecular constructs as a platform for spectral nanoprobes for advanced cellular barcoding in flow cytometry
Original language description
Herein, an advanced bioconjugation technique to synthesize hybrid polymer-antibody nanoprobes tailored for fluorescent cell barcoding in flow cytometry-based immunophenotyping of leukocytes is applied. A novel approach of attachment combining two fluorescent dyes on the copolymer precursor and its conjugation to antibody is employed to synthesize barcoded nanoprobes of antibody polymer dyes allowing up to six nanoprobes to be resolved in two-dimensional cytometry analysis. The major advantage of these nanoprobes is the construct design in which the selected antibody is labeled with an advanced copolymer bearing two types of fluorophores in different molar ratios. The cells after antibody recognition and binding to the target antigen have a characteristic double fluorescence signal for each nanoprobe providing a unique position on the dot plot, thus allowing antibody-based barcoding of cellular samples in flow cytometry assays. This technique is valuable for cellular assays that require low intersample variability and is demonstrated by the live cell barcoding of clinical samples with B cell abnormalities. In total, the samples from six various donors were successfully barcoded using only two detection channels. This barcoding of clinical samples enables sample preparation and measurement in a single tube.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10404 - Polymer science
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Macromolecular Bioscience
ISSN
1616-5187
e-ISSN
1616-5195
Volume of the periodical
24
Issue of the periodical within the volume
2
Country of publishing house
US - UNITED STATES
Number of pages
12
Pages from-to
2300306
UT code for WoS article
001068936800001
EID of the result in the Scopus database
2-s2.0-85172032814