HPMA-based nitroxide radical containing nanoparticles with controlled radical release: detailed physico-chemical characterization
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F25%3A00604458" target="_blank" >RIV/61389013:_____/25:00604458 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11110/25:10492822
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0014305725000151?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0014305725000151?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.eurpolymj.2025.113727" target="_blank" >10.1016/j.eurpolymj.2025.113727</a>
Alternative languages
Result language
angličtina
Original language name
HPMA-based nitroxide radical containing nanoparticles with controlled radical release: detailed physico-chemical characterization
Original language description
This research focuses on the measurement of the release of nitroxide radicals from polymer-based nanoparticles by electron paramagnetic resonance (EPR) and potentiometric titration techniques. Nitroxide radicals such as 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO) are known to modulate oxidative stress by scavenging reactive oxygen species that cause cell damage. It was also found that these biologically active radicals can inhibit tumor growth by inducing apoptosis in cancer cells. Nitroxide radicals, however, cannot be effectively used due to their non-specific accumulation in normal tissue and quick elimination from the body. To address these challenges, we developed pH-responsive nanoparticles based on self-assembly of amphiphilic N-(2-hydroxypropyl)methacrylamide (HPMA)-based copolymers containing TEMPO radicals, covalently attached to the hydrophilic shell by a pH-sensitive hydrazone bond, and compared with pH-unresponsive nanoparticles, where the TEMPO radicals were bound by a physiologically stable amide bond. We thoroughly investigated the nitroxide radicals release from nanoparticles by EPR and potentiometric titration for the first time. TEMPO radicals were almost completely released from the pH-responsive nanoparticles within three hours at pH = 5 at 37 °C, which corresponds to tumor tissue, while only quite slow release of radicals was observed at physiological pH within 24 h. The results show that nitroxide radicals containing HPMA-based nanoparticles could be potentially used either for tumor visualization or as smart prodrugs to enhance nitroxide radicals’ therapeutic efficacy and safety.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10404 - Polymer science
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
European Polymer Journal
ISSN
0014-3057
e-ISSN
1873-1945
Volume of the periodical
225
Issue of the periodical within the volume
14 February
Country of publishing house
GB - UNITED KINGDOM
Number of pages
10
Pages from-to
113727
UT code for WoS article
001411394500001
EID of the result in the Scopus database
2-s2.0-85214333024