Catalytic degradation of N-acyl-homoserine lactone using a copper complex of a TACN derivative: implications for quorum sensing interference
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F25%3A00640885" target="_blank" >RIV/61389013:_____/25:00640885 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/25:10510299
Result on the web
<a href="https://pubs.rsc.org/en/content/articlelanding/2025/dt/d5dt01612f" target="_blank" >https://pubs.rsc.org/en/content/articlelanding/2025/dt/d5dt01612f</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1039/D5DT01612F" target="_blank" >10.1039/D5DT01612F</a>
Alternative languages
Result language
angličtina
Original language name
Catalytic degradation of N-acyl-homoserine lactone using a copper complex of a TACN derivative: implications for quorum sensing interference
Original language description
Medical treatment of bacterial biofilms represents a significant challenge, not only in the case of implant-associated infections but also in chronic inflammation of soft tissues. Therefore, further research is crucial to develop strategies that prevent biofilm formation at early stages, where quorum sensing (QS) signalling molecules that facilitate bacterial communication play a key role. In this study, we investigated the catalytic properties of an N-substituted triazacyclononane-copper(II) complex (Cu(II)-iPr2TACN), which mimicked the enzymatic activity of an acylase in the hydrolysis reaction of N-acyl-homoserine lactone (AHL), disrupting the QS in the bacterial biofilm as a result. Specifically, we focused on kinetic experiments involving the degradation of newly developed model AHLs with an acyl group derived from azobenzene as a chromophore label, enabling UV-VIS detection. ESI-MS was employed to identify the degradation products. Our results demonstrated sufficient hydrolytic activity against the model signalling molecules within relatively short time intervals under body temperature, blood pH and inflammation site conditions, with disruption occurring via lactone ring cleavage followed by further hydrolysis of the acyl fragment of the amide. To our knowledge, this is the first use of such complexes to degrade quorum sensing molecules. In addition, a novel UV-VIS active azodye chromophore-containing model substrate of a quorum sensing molecule was established for quorum quenching degradation studies and critically compared to other natural and synthetic quorum sensing molecules.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10401 - Organic chemistry
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Dalton Transactions
ISSN
1477-9226
e-ISSN
1477-9234
Volume of the periodical
54
Issue of the periodical within the volume
43
Country of publishing house
GB - UNITED KINGDOM
Number of pages
15
Pages from-to
16106-16120
UT code for WoS article
001592180000001
EID of the result in the Scopus database
2-s2.0-105020799935