TYRAY-functionalized alginate bioinks for 3D bioprinting support stem cell culture and endothelial network formation
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F25%3A00643019" target="_blank" >RIV/61389013:_____/25:00643019 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14110/25:00143608 RIV/65269705:_____/25:00083077 RIV/00159816:_____/25:00082287
Result on the web
<a href="https://pubs.acs.org/doi/10.1021/acsbiomaterials.5c01132" target="_blank" >https://pubs.acs.org/doi/10.1021/acsbiomaterials.5c01132</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/acsbiomaterials.5c01132" target="_blank" >10.1021/acsbiomaterials.5c01132</a>
Alternative languages
Result language
angličtina
Original language name
TYRAY-functionalized alginate bioinks for 3D bioprinting support stem cell culture and endothelial network formation
Original language description
3D bioprinting is transforming tissue engineering by enabling spatial arrangement of cells and cell aggregates within supportive hydrogels. Among available materials, alginate remains widely used for its biocompatibility, printability, and cost-effectiveness. However, its bioinert nature and lack of adhesive moieties restrict its capacity to support essential processes like cell adhesion, migration, and proliferation. In this study, we propose a comprehensive approach to enhance alginate hydrogels focusing on stem, stromal, and endothelial cell types to support extended growth and vascular network formation. Key innovations include the incorporation of the TYRAY peptide in 3D alginate hydrogels─its first application in this context─to promote cell adhesion and migration, accompanied by Ca(OH)2-modified surfaces for stable hydrogel anchoring and an ultrasonic mist cross-linking to preserve 3D structure fidelity. Functionalization with the TYRAY peptide significantly enhanced cell proliferation, promoted multicellular spheroid fusion, and supported endothelial network development in comparative culture setting. Together, these findings establish this defined, xeno-free alginate system as a versatile bioink material suitable for 3D culture and bioprinting applications.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10404 - Polymer science
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
ACS Biomaterials Science & Engineering
ISSN
2373-9878
e-ISSN
2373-9878
Volume of the periodical
11
Issue of the periodical within the volume
12
Country of publishing house
US - UNITED STATES
Number of pages
16
Pages from-to
7368-7383
UT code for WoS article
001619486600001
EID of the result in the Scopus database
2-s2.0-105024260754