Membrane damage and active but nonculturable state in liquid cultures of Escherichia coli treated with an atmospheric pressure plasma jet

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389021%3A_____%2F15%3A00444287" target="_blank" >RIV/61389021:_____/15:00444287 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/j.bioelechem.2014.08.018" target="_blank" >http://dx.doi.org/10.1016/j.bioelechem.2014.08.018</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.bioelechem.2014.08.018" target="_blank" >10.1016/j.bioelechem.2014.08.018</a>

Result language

angličtina

Original language name

Membrane damage and active but nonculturable state in liquid cultures of Escherichia coli treated with an atmospheric pressure plasma jet

Original language description

Electrical discharge plasmas can efficiently inactivate various microorganisms. Inactivation mechanisms caused by plasma, however, are not fully understood because of the complexity of both the plasma and biological systems.We investigated plasma-inducedinactivation of Escherichia coli in water and mechanisms by which plasma affects bacterial cell membrane integrity.Atmospheric pressure argon plasma jet generated at ambient air in direct contact with bacterial suspension was used as a plasma source.Wedetermined significantly lower counts of E. coli after treatment by plasma when they were assayed using a conventional cultivation technique than using a fluorescence-based LIVE/DEAD staining method,which indicated that bacteria may have entered the viable-but-nonculturable state (VBNC).We did not achieve resuscitation of these non-culturable cells, however,we detected their metabolic activity through the analysis of cellular mRNA,which suggests that cells may have been rather in the

Czech name

—

Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

<a href="/en/project/LD14080" target="_blank" >LD14080: Chemical processes initiated by electrical discharge plasma in aqueous liquids for new applications</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Bioelectrochemistry

ISSN

1567-5394

e-ISSN

—

Volume of the periodical

103

Issue of the periodical within the volume

June

Country of publishing house

CH - SWITZERLAND

Number of pages

8

Pages from-to

7-14

UT code for WoS article

000351977000003

EID of the result in the Scopus database

2-s2.0-84923363431