On the inactivation of Bacillus subtilis spores by surface streamer discharge in humid air caused by reactive species

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389021%3A_____%2F20%3A00531308" target="_blank" >RIV/61389021:_____/20:00531308 - isvavai.cz</a>

Result on the web

<a href="https://iopscience.iop.org/article/10.1088/1361-6463/ab7cf7" target="_blank" >https://iopscience.iop.org/article/10.1088/1361-6463/ab7cf7</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1088/1361-6463/ab7cf7" target="_blank" >10.1088/1361-6463/ab7cf7</a>

Result language

angličtina

Original language name

On the inactivation of Bacillus subtilis spores by surface streamer discharge in humid air caused by reactive species

Original language description

The inactivation of Bacillus subtilis (ATCC 6633) spores deposited on a filter membrane was studied by using low-temperature plasma produced via surface dielectric barrier discharge. Spore samples were carefully prepared to avoid the formation of cell aggregates, and their inactivation was induced by multiple surface streamer discharge driven in a coplanar dielectric barrier discharge electrode geometry by an amplitude-modulated AC high voltage waveforms in humid air at atmospheric pressure. At a discharge duty cycle of 0.4, the surface dielectric barrier discharge is characterised by an average total power of 1.7 W (power density 1.5 W cm-2 and energy density ∼0.3 Wh l-1) and a low gas temperature of the plasma filaments of about 320 K. The spores were exposed by placing a sample holder at a fixed distance of 3 mm from the electrode surface covered by plasma filaments. Particular attention was paid to identifying sporicidal agents employed in the process of inactivation. Since treated samples did not come into direct contact with the streamer filaments and excessive heating was excluded thanks to the low energy density, our results indicate that the spores were inactivated mainly by reactive oxygen and nitrogen species such as O3, H2O2 and NO2-. Discharge-induced damage of the spore structure was evidenced via the detection of dipicolinic acid and leaking of intracellular components. We therefore conclude that B. subtilis spores were inactivated chemically, probably due to failure of the coat structure or membrane of the spore.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10305 - Fluids and plasma physics (including surface physics)

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Physics D-Applied Physics

ISSN

0022-3727

e-ISSN

—

Volume of the periodical

53

Issue of the periodical within the volume

24

Country of publishing house

GB - UNITED KINGDOM

Number of pages

12

Pages from-to

245203

UT code for WoS article

000528541600001

EID of the result in the Scopus database

2-s2.0-85084734532