Leucine modifications by He/O2 plasma treatment in phosphate-buffered saline: Bactericidal effects and chemical characterization
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389021%3A_____%2F21%3A00555846" target="_blank" >RIV/61389021:_____/21:00555846 - isvavai.cz</a>
Result on the web
<a href="https://iopscience.iop.org/article/10.1088/1361-6463/ac252e" target="_blank" >https://iopscience.iop.org/article/10.1088/1361-6463/ac252e</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1088/1361-6463/ac252e" target="_blank" >10.1088/1361-6463/ac252e</a>
Alternative languages
Result language
angličtina
Original language name
Leucine modifications by He/O2 plasma treatment in phosphate-buffered saline: Bactericidal effects and chemical characterization
Original language description
We studied the chemical and bactericidal properties of plasma-modified amino acid leucine (Leu) treated in phosphate-buffered saline (PBS) using an atmospheric-pressure plasma jet operating with He/O2 gas. We determined the initial formation of hypochlorite by the reaction of plasma-supplied O atoms with chloride ions in PBS, followed by the subsequent chlorination of Leu. The principal chemical process involved the formation of Leu monochloramine and Leu dichloramine. The products of plasma-modified Leu further possessed post-discharge reactivity characterized by the decay of Leu chloramines to the secondary products, i.e. isovaleraldehyde, isovaleronitrile, and N-chloromethyl-3-butaldimine. Our study of the effects of Leu on the viability of Escherichia coli bacteria in the plasma-treated PBS solution demonstrated time-and pH-dependent cytotoxic behavior, which were associated with the formation of different reaction products. At shorter treatment times, the addition of Leu hindered the bactericidal properties of plasma-treated PBS caused by the consumption of bactericidal hypochlorite in reaction with Leu and formation of non-bactericidal Leu monochloramine. Acidification to pH = 3 and longer plasma treatment substantially enhanced the bactericidal effects of Leu solutions, which were tentatively assigned to the formation of Leu dichloramine.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10305 - Fluids and plasma physics (including surface physics)
Result continuities
Project
<a href="/en/project/GA19-25026S" target="_blank" >GA19-25026S: Characteristics of chemically reactive liquids produced by non-equilibrium plasma</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Physics D-Applied Physics
ISSN
0022-3727
e-ISSN
1361-6463
Volume of the periodical
54
Issue of the periodical within the volume
50
Country of publishing house
GB - UNITED KINGDOM
Number of pages
11
Pages from-to
505206
UT code for WoS article
000701256000001
EID of the result in the Scopus database
2-s2.0-85116879960