Sorting of mitotic chromosomes in common wheat (Triticum aestivum L.) using flow cytometry
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F01%3A00100780" target="_blank" >RIV/61389030:_____/01:00100780 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Sorting of mitotic chromosomes in common wheat (Triticum aestivum L.) using flow cytometry
Original language description
The aim of this study was to develop an improved procedure for preparation of chromosome suspensions, and to evaluate the potential of flow cytometry for chromosome sorting in wheat. Suspensions of intact chromosomes were prepared by mechanical homogenization of synchronized root tips after mild fixation with formaldehyde. Histograms of relative fluorescence intensity (flow karyotypes) obtained after the analysis of DAPI-stained chromosomes were characterized and the chromosome content of all peaks on wheat flow karyotype was determined for the first time. Only chromosome 3B could be discriminated on flow karyotypes of wheat lines with standard karyotype. Remaining chromosomes formed three composite peaks and could be sorted only as groups. Chromosome3B could be sorted at purity >95% as determined by microscopic evaluation of sorted fractions that were labeled using C-PRINS with primers for GAA microsatellites and for Afa repeats, respectively.
Czech name
Sorting of mitotic chromosomes in common wheat (Triticum aestivum L.) using flow cytometry
Czech description
Sorting of mitotic chromosomes in common wheat (Triticum aestivum L.) using flow cytometry.
Classification
Type
C - Chapter in a specialist book
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/IBS5038104" target="_blank" >IBS5038104: Molecular cytogenetic and cytometric methods for breeding of grasses and trifolium</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2001
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Book/collection name
Wheat in a Global Environment
ISBN
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Number of pages of the result
6
Pages from-to
531-536
Number of pages of the book
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Publisher name
Kluwer
Place of publication
Dordrecht
UT code for WoS chapter
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