Sorting of mitotic chromosomes in common wheat (Triticum aestivum L.) using flow cytometry.

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F01%3A00001274" target="_blank" >RIV/61989592:15310/01:00001274 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Sorting of mitotic chromosomes in common wheat (Triticum aestivum L.) using flow cytometry.
Original language description
The analysis of wheat genome could be simplified by its separation into individual chromosome and/or chromosome arms. The aim of this study was to develop an improved procedure for preparation of chromosome suspensions and to evaluate the potential of flow cytometry for chromosome sorting in wheat. Suspensions of intact chromosomes were prepared by mechanical homogenisation of synchronized root tip meristems after a mild formaldehyde fixation. The analysis of DAPI-staining chromosome suspensions resulted in flow karyotypes containing three composite peaks representing groups of chromosomes. In addition, a peak that represented chromosome 3B was well resolved. This chromosome could be sorted at high purity. Sorting of other chromosomes was not possible.The work is in progress to assess the suitability of various chromosome stocks to overcome this problem. For instance using chromosome translocation lines it was possible to sort chromosome 5BL/7BL. Furthermore, some ditelosomic lines wer
Czech name
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Czech description
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Project
<a href="/en/project/GV521%2F96%2FK117" target="_blank" >GV521/96/K117: Novel approaches for effective analysis and mapping of genomes in cultivated plants</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2001
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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