DNA damage induced by hydrogen peroxide in cultured tobacco cells is dependent on the cell growth stage.
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F02%3A56023001" target="_blank" >RIV/61389030:_____/02:56023001 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
DNA damage induced by hydrogen peroxide in cultured tobacco cells is dependent on the cell growth stage.
Original language description
The level of hydrogen peroxide (HaOaMnduced genomic DNA damage measured by the Comet assay in tobacco suspension cells (TX1) increased as a function of the age of the culture. After treatment of TX1 cells with 15 mM H2O2, the average ( S.E.) median tailmoment value was only 4.85 1.00 |xm in nuclei isolated from 2-day-old cells compared to 72.33 1.40 (xm in nuclei isolated from 12-day-old cells. By contrast, nuclei first isolated from 2 and 12-day-old cells and then treated with HfeC^, expressed the same level of DNA damage. The activity of catalases was markedly higher in 2-day-old TX1 cells compared to 12-day-old cells. The results indicate that the reaction of the H2O2 with nuclear DNA is modified by the presence of the plant cell wall, and enzymesand macromolecules present in the cytosol, and is not connected with changes in the nuclear DNA sensitivity during cell suspension growth. ř 2002 Elsevier Science B.V. All rights reserved.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA521%2F02%2F0400" target="_blank" >GA521/02/0400: Genotoxicity of environmental mutagens in higher plants detected by molecular biological methods</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2002
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Mutation Research
ISSN
1383-5718
e-ISSN
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Volume of the periodical
514
Issue of the periodical within the volume
N/A
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
6
Pages from-to
147-152
UT code for WoS article
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EID of the result in the Scopus database
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