Potato virus X induces DNA damage in leaf nuclei of the host plant Nicotiana tabacum L. var. xanthi

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F14%3A10289034" target="_blank" >RIV/00216208:11310/14:10289034 - isvavai.cz</a>

Alternative codes found

RIV/61389030:_____/14:00438179

Result on the web

<a href="http://dx.doi.org/10.1007/s10535-014-0448-z" target="_blank" >http://dx.doi.org/10.1007/s10535-014-0448-z</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10535-014-0448-z" target="_blank" >10.1007/s10535-014-0448-z</a>

Result language

angličtina

Original language name

Potato virus X induces DNA damage in leaf nuclei of the host plant Nicotiana tabacum L. var. xanthi

Original language description

We employed the comet assay (single cell gel electrophoresis) to evaluate induced DNA damage in nuclei isolated from tobacco leaves (Nicotiana tabacum var. xanthi) inoculated with Potato virus X (PVX). The highest DNA damage, expressed by the tail momentvalue, was observed in the inoculated leaves and decreased in the 1(st) to 4(th) systemic leaves. DNA damage increased with the time after the inoculation (from day 3 to day 21) and was higher in nuclei isolated from a part of the leaf at the petiole compared to nuclei isolated from the leaf tip. A Pearson moment correlation (r = 0.94) between the induced DNA damage and the PVX titres expressed by ELISA absorbance values was observed. The PVX infection did not induce a significant increase in the rateof somatic mutations evaluated by appearance of dark green, yellow, and double green/yellow sectors on the heterozygous pale green leaves of N. tabacum var. xanthi.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

<a href="/en/project/GAP501%2F12%2F1761" target="_blank" >GAP501/12/1761: Impact of different topology of heterologous antigenic determinants in PVX-based plant viral vector on their expression level and immunogenicity</a><br>

Continuities

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biologia Plantarum

ISSN

0006-3134

e-ISSN

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Volume of the periodical

58

Issue of the periodical within the volume

4

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

5

Pages from-to

783-787

UT code for WoS article

000344596800023

EID of the result in the Scopus database

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