Evaluation of DNA damage and mutagenicity induced by lead in tobacco plants

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F08%3A00307201" target="_blank" >RIV/61389030:_____/08:00307201 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Evaluation of DNA damage and mutagenicity induced by lead in tobacco plants

Original language description

Tobacco (Nicotiana tabacum L. var. xanthi) seedlings were treated with aqueous solutions of lead nitrate (Pb2+) at concentrations ranging from 0.4 mM to 2.4 mM for 24 h and from 25 ?M to 200 ?M for 7 days. The DNA damage measured by the comet assay was high in the root nuclei, but in the leaf nuclei a slight but significant increase in DNA damage could be demonstrated only after a 7-day treatment with 200 ?M Pb2+. In tobacco plants growing for 6 weeks in soil polluted with Pb2+ severe toxic effects, expressed by the decrease in leaf area, and a slight but significant increase in DNA damage were observed. The tobacco plants with increased levels of DNA damage were severely injured and showed stunted growth, distorted leaves and brown root tips. The frequency of somatic mutations in tobacco plants growing in the Pb2+-polluted soil did not significantly increase. Analytical studies by inductively coupled plasma optical emission spectrometry demonstrate that after a 24-h treatment of tobac

Czech name

Hodnocení poškození DNA a mutagenity po aplikaci olova u rostlin tabaku

Czech description

Semenáčky tabáku (Nicotiana tabacum var. xanthi) byly ovlivněny roztoky Pb (NO3)2 po dobu 24 h a 7 dní. Průkazné zvýšení poškození DNA, měřeno kometovým testem, bylo prokázáno pouze v kořenech semenáčku. Po kultivaci semenáčků tabáku v pudě kontaminovanéPb (NO3)2 po dobu 6 týdnů bylo zvýšení poškození DNA vždy spojeno se silnou toxicitou (inhibice růstu semenáčků, nahnědlé a zdeformované listy). Zvýšení frekvence somatických mutací nebylo prokázáno. Zvýšení poškozené DNA je pravděpodobně podmíněné nekrotickou fragmentací DNA.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/GA521%2F05%2F0500" target="_blank" >GA521/05/0500: Induced DNA damage, mutagenicity, homologous recombination and apoptosis as endpoints for monitoring effects of pollutants in tobacco and potato</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)<br>V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Publication year

2008

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Mutation Research - Genetic Toxicology and Environmental Mutagenesis

ISSN

1383-5718

e-ISSN

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Volume of the periodical

652

Issue of the periodical within the volume

2

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

5

Pages from-to

186-190

UT code for WoS article

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EID of the result in the Scopus database

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