Intracellular phosphorylation of benzyladenosine is related to apoptosis induction in tobacco BY-2 cells

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F03%3A00104352" target="_blank" >RIV/61389030:_____/03:00104352 - isvavai.cz</a>

Alternative codes found

RIV/62156489:43210/03:00003692

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Intracellular phosphorylation of benzyladenosine is related to apoptosis induction in tobacco BY-2 cells

Original language description

Treatment of tobacco BY-2 cells with micromolar concentration of benzyladenosine ([9R]BA) resulted in the loss of cell viability in a time- and concentration-dependent manner. Cell death induced by [9R]BA exhibited typical apoptotic hallmarks including cell shrinkage, chromatin condensation and degradation of nuclear DNA to characteristic high molecular weight (HMW) as well as nucleosomal size fragments. Externally added [9R]BA was very rapidly and almost quantitatively phosphorylated within BY-2 cells.Accumulation of [9R]BA-monophosphate was accompanied by massive production of endogenous reactive oxygen species (ROS), intracellular ATP depletion, and these events were followed by the loss of cell viability. Inhibition of intracellular phosphorylation of [9R]BA by adenosin kinase inhibitor, 5'-amino-5'-deoxyadenosine (AdAs), diminished ROS production, ATP depletion, and consequently prevented cells from death.

Czech name

Intracellular phosphorylation of benzyladenosine is related to apoptosis induction in tobacco BY-2 cells

Czech description

Intracellular phosphorylation of benzyladenosine is related to apoptosis induction in tobacco BY-2 cells.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

ED - Physiology

OECD FORD branch

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Project

<a href="/en/project/LN00A081" target="_blank" >LN00A081: Signaling pathways in plants</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2003

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Plant Cell and Environment

ISSN

0140-7791

e-ISSN

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Volume of the periodical

26

Issue of the periodical within the volume

10

Country of publishing house

GB - UNITED KINGDOM

Number of pages

13

Pages from-to

1723-1735

UT code for WoS article

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EID of the result in the Scopus database

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