Analysis and sorting of rye (Secale cereale L.) chromosomes using flow cytometry.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F03%3A56033020" target="_blank" >RIV/61389030:_____/03:56033020 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Analysis and sorting of rye (Secale cereale L.) chromosomes using flow cytometry.

Original language description

Procedures for chromosome analysis and sorting using flow cytometry were developed for rye (Secale cereale L.). Chromosome 1R could be discriminated on a flow caryotype. Remaining rye chromosomes (2R-7R) could be discriminated and sorted from individualwheat-rye addition lines. Flow sorted chromosomes were identified by fluorescence in situ hybridization. PCR with chromosome-specific primers confirmed the identity of sorted fractions and indicated suitability of sorted chromosomes for physical mapping.The possibility to sort large numbers of chromosomes opens a way for the construction of large-insert chromosome-specific DNA libraries in rye.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2003

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Genome

ISSN

0831-2796

e-ISSN

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Volume of the periodical

46

Issue of the periodical within the volume

N/A

Country of publishing house

CA - CANADA

Number of pages

13

Pages from-to

893-905

UT code for WoS article

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EID of the result in the Scopus database

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