Hexokinases of tobacco leaves: changes in the cytosolic and non-cytosolic isozyme complexes induced by tobacco mosaic virus infection.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F03%3A56033043" target="_blank" >RIV/61389030:_____/03:56033043 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Hexokinases of tobacco leaves: changes in the cytosolic and non-cytosolic isozyme complexes induced by tobacco mosaic virus infection.

Original language description

Changes in the cytosolic (soluble) and the non-cytosolic (particulate) isozyme composition of hexokinases were studied by ion exchange chromatography after subcellular fractionation in TMV infected tobacco leaves. Three main isozyme complexes were obtained: one particulate fraction (particulate hexokinase) and two soluble fractions (soluble hexokinase and soluble fructokinase). The total fructokinase activities were nearly twice higher than the total glucose activities. The total particulate glucokinaseand fructokinase activity were increased to 120% in TMV infected tissue. The sum of soluble glucokinase activity was increased to 175% and of fructokinase activity to 131% in TMV infected tissue. The isozymes isolated both from the healthy control and TMV-infected leaves had the similar elution profiles, displayed Michaelis-Menten kinetics, showed the identical profiles of pH optima and were Mg2+ dependent with the highest enzyme activity at equimolar Mg2+ and ATP concentration.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

<a href="/en/project/GA522%2F02%2F0708" target="_blank" >GA522/02/0708: The role of chloroplasts in mechanisms involved in replication and translation of viral-RNA in connection with host resistance</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2003

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biologia plantarum

ISSN

0006-3134

e-ISSN

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Volume of the periodical

47

Issue of the periodical within the volume

3

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

7

Pages from-to

413-419

UT code for WoS article

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EID of the result in the Scopus database

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