Use of Two-Dimensional Fluorescence Spectroscopy for Monitoring of the Effect of Dimethyl Sulfoxide in the Growth and Viability of Immobilized Plant Cells.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F03%3A56033044" target="_blank" >RIV/61389030:_____/03:56033044 - isvavai.cz</a>

Alternative codes found

RIV/67985858:_____/03:56033044 RIV/61388963:_____/03:56033044

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Use of Two-Dimensional Fluorescence Spectroscopy for Monitoring of the Effect of Dimethyl Sulfoxide in the Growth and Viability of Immobilized Plant Cells.

Original language description

The growth and viability of tobacco cells (Nicotiana tabacum L.) immobilized in alginate or pectate were' monitored during their cultivation by using two-dimensional fluorescence spectroscopy (2-D FS). The cell growth was followed via the fluorescence ofamino acids in proteins. The correlation between the tryptophan fluorescence and the cell biomass inside the alginate beads was verified by comparison with the dry weight of the cells. The determination of biomass content or cell viability by measurement of the intensity of NAD(P)H fluorescence was found unsuitable. Cell viability was estimated by determination of cell esterase activity using fluorescein diacetate as a fluorogenic substrate. The fluorescence intensities of both fluorophores, tryptophanand fluorescein, were determined by scanning a 2-D FS spectrum of intact beads in front face cuvette. Using this technique the effect of organic solvent, dimethyl sulfoxide, on the growth and metabolic activities of cells within the bead

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2003

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Chemical industry

ISSN

0354-7531

e-ISSN

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Volume of the periodical

57

Issue of the periodical within the volume

12

Country of publishing house

HR - CROATIA

Number of pages

4

Pages from-to

632-635

UT code for WoS article

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EID of the result in the Scopus database

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