Monitoring of the Effect of Dimethyl Sulfoxide on the Growth and Viability of Immobilized Plant Cells Using Two-dimensional Fluorescence Spectroscopy.

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F03%3A56033145" target="_blank" >RIV/61389030:_____/03:56033145 - isvavai.cz</a>
Alternative codes found
RIV/67985858:_____/03:56033145 RIV/61388963:_____/03:56033145
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Monitoring of the Effect of Dimethyl Sulfoxide on the Growth and Viability of Immobilized Plant Cells Using Two-dimensional Fluorescence Spectroscopy.
Original language description
Using two-dimensional fluorescence spectroscopy, the growth of tobacco cells immobilized in alginate and pectate was monitored during the cultivation of the beads via the fluorescence of proteins (especially of tryptophan). Cell viability was followed bydetermination of their esterase activity using fluorescein diacetate as a substrate. This technique enables to measure the fluorescence of two fluorophores simultaneously. The effect of several stresses, (namely high and low temperature and the presenceof organic solvent dimethylsulfoxide) was followed. Suitability of this technique to monitor the metabolic activities of cells within the beads was demonstrated.
Czech name
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Czech description
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Project
<a href="/en/project/OC%20840.20" target="_blank" >OC 840.20: Immobilization as a tool for plant physiology studies</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2003
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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