Effect of exogenous cytokinins, auxins and adenine on cytokinin N-glucosylation and cytokinin oxidase/dehydrogenase activity in de-rooted radish seedlings

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F04%3A00104354" target="_blank" >RIV/61389030:_____/04:00104354 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Effect of exogenous cytokinins, auxins and adenine on cytokinin N-glucosylation and cytokinin oxidase/dehydrogenase activity in de-rooted radish seedlings

Original language description

The role of cytokinin N-glucosylation and degradation by cytokinin oxidase/dehydrogenase (CKX, EC 1.5.99.12) in response to application of exogenous auxins (2,4-dichlorophenoxyacetic acid [2,4-D] and ?-naphthaleneacetic acid [NAA]) and cytokinins (N6-benzyladenine [BA] and trans-zeatin [Z]) was investigated in de-rooted seedlings of Raphanus sativus L. cv. Rampouch. Both auxins applied for 24 h at 1 and 10 ?M concentration increased N-glucosylation of exogenously applied [3H]dihydrozeatin (DHZ) by up to20%. The level of endogenous 7N-glucosides (of Z, isopentenyladenine [iP] and DHZ) was increased by 2,4-D and NAA at 10 ?M concentration by 28 and 23%, respectively, the level of Z being decreased by 90 and 59%, respectively. 2,4-D and NAA suppressed CKX activity ca. by half. Exogenous cytokinins Z and BA applied at 1 and 10 ?M concentration stimulated 7N-glucosylation of [3H]DHZ (by up to 40%). BA both at 1 and 10 ?M, increased the level of endogenous Z by up to 35% and that of 7N-g...

Czech name

Vliv exogenních cytokininů, auxinů a adeninu na N-glukosylaci cytokininů a aktivitu cytokinin oxidázy/dehydrogenázy v klíčních rostlinách ředkvičky

Czech description

Vliv aplikace exogenních auxinů (2,4-dicholorofenoxyoctové kyseliny [2,4-D] and ?-naftyloctové kyseliny [NAA]) a cytokininů (N6-benzyladeninu [BA] a trans-zeatinu [Z]) na N-glukosylaci cytokininů a jejich degradaci cytokinin oxidázou/dehydrogenázou (CKX,EC 1.5.99.12) byl sledován v nadzemních částech klíčních rostlin ředkvičky Raphanus sativus cv. Rampouch. Oba auxiny (v koncentraci 1 and 10 ?M) vedly po 24 hodinové inkubaci ke zvýšení N-glukosylace exogenního [3H]dihydrozeatinu (DHZ) až o 20%. Hladinaendogenních 7N-glukosidů (Z, isopentenyladeninu [iP] a DHZ) byla zvýšena po aplikaci 10 ?M 2,4-D a 10 ?M NAA o 28%, respektive 23%. Přičemž hladina endogenního Z byla snížena o 90%, respektive 59%. 2,4-D a NAA snížily aktivitu CKX zhruba na polovinu. Exogenní cytokininy Z a BA aplikované v koncentraci 1 a 10 ?M stimulovaly 7N-glukosylaci [3H]DHZ (až o 40%). BA (1 ?M a 10 ?M) vedl ke zvýšení hladiny endogenního Z až o 35% a 7N-glukosidů až o 27%. Aplikace BA silně stimulovala aktivi...

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GE - Plant cultivation

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Plant Growth Regulation

ISSN

0167-6903

e-ISSN

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Volume of the periodical

44

Issue of the periodical within the volume

1

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

9

Pages from-to

15-23

UT code for WoS article

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EID of the result in the Scopus database

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