Transient expression of heterologous model gene in plants using Potato virusX-based vector
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F04%3A00109162" target="_blank" >RIV/61389030:_____/04:00109162 - isvavai.cz</a>
Result on the web
—
DOI - Digital Object Identifier
—
Alternative languages
Result language
angličtina
Original language name
Transient expression of heterologous model gene in plants using Potato virusX-based vector
Original language description
To optimize the efficiency of expression of foreign proteins using Potato virus X (PVX) -- based vector, the gene for the coat protein (CP) of other virus (Potato virusA, PVA) was cloned into the vector, propagated in E. coli and subsequently inoculatedor agroinfected into the host plants. Host range studies showed that the best host plant is N. benthamiana. By means of RT PCR the presence and the stability of the construct were tested. Both ELISA and Western blot analysis were applicable for expressedprotein detection. Expression level of PVA CP achieved approximately 5--10 per mille of total soluble proteins. The results demonstrated that agroinfection is the most suitable method for the propagation of our model gene using PVX--based vectors.
Czech name
Transient expression of heterologous model gene in plants using Potato virusX-based vector
Czech description
Transient expression of heterologous model gene in plants using Potato virusX-based vector.
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
—
Result continuities
Project
<a href="/en/project/GA310%2F00%2F0381" target="_blank" >GA310/00/0381: Production of human papillomavirus proteins in transgenic plants: an attempt to develop edible vaccines</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2004
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Plant Cell, Tissue and Organ Culture
ISSN
0167-6857
e-ISSN
—
Volume of the periodical
79
Issue of the periodical within the volume
2
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
6
Pages from-to
147-152
UT code for WoS article
—
EID of the result in the Scopus database
—