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High content of endogenous cytokinins stimulates activity of enzymes and proteins involved in stress response in Nicotiana tabacum

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F04%3A00109757" target="_blank" >RIV/61389030:_____/04:00109757 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    High content of endogenous cytokinins stimulates activity of enzymes and proteins involved in stress response in Nicotiana tabacum

  • Original language description

    Transgenic Pssu-ipt tobacco with elevated content of endogenous cytokinins grown under in vitro conditions exhibited elevated activities of antioxidant enzymes (i.e. catalase, ascorbate peroxidase, guaiacol and syringaldazine peroxidase, glutathione reductase) and some of enzymes involved in anaplerotic pathways such as glucose-6-phosphate dehydrogenase, glycolate oxidase, NADP-malic enzyme, NADP-isocitrate dehydrogenase, and glutamate dehydrogenase compared to control non-transgenic SR1 tobacco. Higheractivities of peroxidases, NADP-malic enzyme, and glutamate dehydrogenase were maintained in transgenic grafts after several weeks of the growth under ex vitro conditions, while transgenic rooted plants showed only the increase in activity of glycolateoxidase compared to control non-transformed tobacco. Total activities of superoxide dismutase were lower in both types of Pssu-ipt tobacco contrary to controls under both growth conditions. The presence of PR-1 protein and proteins wit...

  • Czech name

    Vysoký obsah endogenních cytokininů stimuluje aktivity enzymů a proteinů účastnících se odpovědí na stres v Nicotiana tabacum

  • Czech description

    Transgenní Pssu-ipt tabák se zvýšeným obsahem endogenních cytokininů, pěstovaný v in vitro podmínkách, vykazuje ve srovnání s netransgenním tabákem zvýšené aktivity antioxidačních enzymů (tj. katalasy, askorbátperoxidasy, quaiacol a syringaldazinperoxidasy, glutationreduktasy) a některých anaplerotických enzymů jako je glukoza-6-fosfátdehydrogenasa, glykolátoxidasa, NADP-jablečný enzym, NADP-isocitrátdehydrogenasa a glutamátdehydrogenasa. Vyšší aktivity peroxidas, NADP-jablečného enzymu a glutamátdehydrogenasy zůstaly u transgenních roubů zachovány i po několika týdnech kultivace v ex vitro podmínkách, zatímco transgenní kořenící rostliny vykazovaly pouze zvýšenou aktivitu glykolátoxidasy ve srovnání s kontrolou. Celkové aktivity superoxiddismutasy byly nižší u obou typů transgenního tabáku než u kontrol. V podmínkách in vitro a ex vitro byla zjištěna přítomnost PR-1 proteinů a proteinů se zvýšenou aktivitou chitinas v extracelulárním tekutině.

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    ED - Physiology

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2004

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    PLANT CELL TISSUE AND ORGAN CULTURE

  • ISSN

    0167-6857

  • e-ISSN

  • Volume of the periodical

    79

  • Issue of the periodical within the volume

    2

  • Country of publishing house

    NL - THE KINGDOM OF THE NETHERLANDS

  • Number of pages

    11

  • Pages from-to

    169-179

  • UT code for WoS article

  • EID of the result in the Scopus database