Papaya ringspot virus coat protein gene for antigen presentation Escherichia coli
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F06%3A00098694" target="_blank" >RIV/61389030:_____/06:00098694 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Papaya ringspot virus coat protein gene for antigen presentation Escherichia coli
Original language description
The coat protein (CP) of Papaya ringspot virus (PRSV) was analyzed for presentation of the antigenic peptide of animal virus, Canine parvovirus (CPV), in Escherichia coli (E. coli). The 45 nucleotides fragment coding for the 15-aa peptide epitope of theCPV-VP2 protein was either inserted into the PRSV-cp gene at the 5', 31 ends, both 5' and 3' ends or substituted into the 3' end of the PRSV cp gene. Each of the chimeric PRSV cp genes was cloned into the pRSET B vector under the control of the T7 promoter and transformed into E. coli. The recombinant coat proteins expressed from different chimeric PRSV-cp genes were purified and intraperitoneally injected into mice. All of the recombinant coat proteins showed strong immunogenicity and stimulate mice immune response. The recombinant coat proteins containing the CPV epitope insertion at the C terminus and at both N and C termini elicited ten times higher specific antisera in immunized mice compared with the other two recombinant coat pro
Czech name
Papaya ringspot virus coat protein gene for antigen presentation Escherichia coli
Czech description
Papaya ringspot virus coat protein gene for antigen presentation Escherichia coli.
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EF - Botany
OECD FORD branch
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Result continuities
Project
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Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)<br>V - Vyzkumna aktivita podporovana z jinych verejnych zdroju
Others
Publication year
2006
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Biochemistry and Molecular Biology
ISSN
1225-8687
e-ISSN
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Volume of the periodical
39
Issue of the periodical within the volume
1
Country of publishing house
KR - KOREA, REPUBLIC OF
Number of pages
6
Pages from-to
16-21
UT code for WoS article
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EID of the result in the Scopus database
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