DNA vaccines based on chimeric potyvirus-like particles carrying HPV16 E7 peptide (aa 44-60)

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F05%3A00028430" target="_blank" >RIV/61389030:_____/05:00028430 - isvavai.cz</a>

Alternative codes found

RIV/00023736:_____/05:00006012

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

DNA vaccines based on chimeric potyvirus-like particles carrying HPV16 E7 peptide (aa 44-60)

Original language description

Vaccine strategies for the treatment of humen papillomavirus-induced cervical cancer are based maily on the humen papillomavirus 16 E7 (HPV 16 E7) oncoprotein. The immunogenicity of the E7 gene has been enhanced by its fusion to many different genes. Here, we linked a short sequence coding for the E7 peptide (aa 44-60) containing immunodominant epitopes for B and T cells to the 3´ end of the gene coding for the whole coat protein (CP) of the potyvirus, potato virus A, and its deleted form (CPdel) with ashort C-terminal deletion of 5 amino acids (LGVKG). CP-E7 and CPdel-E7 fusion proteins, just like CP alone, spotaneously assembled into virus-like particles in both procaryotic and eucaryotic cells. The CP-E7 and CPdel-7 induced slightly stronger E7-specific cytotoxin T-lymphocyte response than the whole E7 gene, although they were still lower than those elicited by the previously consctructed fusion gene, Sig/E7GGG/LAMP-1. The E-7 and CP-specific antibody responses were not detec...

Czech name

DNA vakcinace založená na chimérických viru podobných částicích polyvirů nesoucích HPV 16 E7 peptid (aa44-66)

Czech description

Geny pro obalový protein A viru bramboru (PVACP) a pro různé kombinace PVACP + epitopy ze strukturních a nestrukturních proteinů (E7 +L2) lidského papilloma viru HPV-16 byly vloženy do vektorového systému založeného X viru bramboru (PVX), kde tvořily viru podobné částice. Uvedené konstrukty byly použity k vakcinaci laboratorních zvířat, kdy protilátková odpověď nebyla detekována, a k DNA vakcinaci, kdy se projevila ochrana myší před tumory vyvolanými buněčnou nádorovou linií TC-1.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EI - Biotechnology and bionics

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2005

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Oncology Reports

ISSN

1021-335X

e-ISSN

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Volume of the periodical

14

Issue of the periodical within the volume

4

Country of publishing house

GR - GREECE

Number of pages

9

Pages from-to

1045-1053

UT code for WoS article

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EID of the result in the Scopus database

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