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The architecture of the chloroplast trnH-psbA non-coding region in angiosperms

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F07%3A00099494" target="_blank" >RIV/61389030:_____/07:00099494 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    The architecture of the chloroplast trnH-psbA non-coding region in angiosperms

  • Original language description

    The psbA-trnH intergenic region is among the most variable regions in the angiosperm chloroplast genome. It is a popular tool for plant population genetics and species level phylogenetics and has been proposed as suitable for DNA barcoding studies. Thisregion contains two parts differing in their evolutionary conservation: 1) the psbA 3?UTR (untranslated region) and 2) the psbA-trnH intergenic non-transcribed spacer. We compared the sequence and RNA secondary structure of the psbA 3' UTR across angiosperms and found consensus motifs corresponding to the stem portions of the RNA stem-loop structures and a consensus TTAGTGTATA box. The psbA-trnH spacer exhibited patterns explicable by the independent evolution of large inversions in the psbA 3?UTR and mutational hot spots in the remaining portion of the psbA-trnH spacer. We conclude that a comparison of chloroplast UTRs across a angiosperms offer clues to the identity of putative regulatory elements and information about selective const

  • Czech name

    Architektura nekódující chloroplastové oblasti psbA-trnH u krytosemenných rostlin

  • Czech description

    Intergenová oblast psbA-trnH v chloroplastovém genomu krytosemenných rostlin je velmi variabilní a hojně využívaná při studiu populací. Byla navržena k použití pro ?barcoding?, tedy k určování druhů podle DNA sekvence. Tato oblast obsahuje psbA 3?UTR přepisovaný úsek. Srovnali jsme sekvenci a sekundární strukturu RNA v této oblasti u krytosemenných rostlin a nalezli konzervované motivy odpovídající stonkům vlásenek dvouřetězcové RNA a konsensus sekvenci TTAGTGTATA, která nevykazovala výraznou sekundárnístrukturu. Srovnání chloroplastových UTR pomůže pomoci najít evolučně konzervované elementy a pochopit evoluci nekódujících oblastí chloroplastov0ho genomu.

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EB - Genetics and molecular biology

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/LC06004" target="_blank" >LC06004: Integration of research activities to study the plant genome</a><br>

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)<br>V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Others

  • Publication year

    2007

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Plant Systematics and Evolution

  • ISSN

    0378-2697

  • e-ISSN

  • Volume of the periodical

    268

  • Issue of the periodical within the volume

    1-4

  • Country of publishing house

    AT - AUSTRIA

  • Number of pages

    22

  • Pages from-to

    235-256

  • UT code for WoS article

  • EID of the result in the Scopus database