C-terminal hydrophobic region leads PRSV P3 Protein to endoplasmic reticulum

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F07%3A00307206" target="_blank" >RIV/61389030:_____/07:00307206 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

C-terminal hydrophobic region leads PRSV P3 Protein to endoplasmic reticulum

Original language description

P3 protein is one of the least characterized potyviral proteins in both functions and sub-cellular localization. In this study, we examined the sub-cellular localization of PRSV P3 and its intermediate, P3-6K1 by expressing their GFP fusion proteins in onion epidermal cells. Our results showed that both P3- and P3-6K1 GFP fusion proteins were localized at the endoplasmic reticulum. Deletion analysis indicated that C-terminal of P3 protein contained localization signal, and a 19 amino acids hydrophobic domain from this region was able to target the GFP fusion protein to endoplasmic reticulum. C-terminal of P3 proteins has been suggested to be involved in both viability and pathogenicity of the potyvirus. Therefore, our result suggests that localizationof P3 protein at endoplasmic reticulum is essential for functionality of P3 protein.

Czech name

C-terminální hydrofobní oblast navádí protein PRSV P3 k endoplasmatickému retikulu

Czech description

Technologie exprese fúzního proteinu v epidermálních buňkách cibule byla použita pro studium PRSV proteinu P3-6K1. Tento protein byl lokalizován v endoplasmatickém retikulu, přičemž 19 aminokyselin dlouhá hydrofobní doména na C-konci je zodpovědná za nasměrování proteinu do endoplasmatickému retikula.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)<br>V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Publication year

2007

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Virus Genes

ISSN

0920-8569

e-ISSN

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Volume of the periodical

35

Issue of the periodical within the volume

3

Country of publishing house

US - UNITED STATES

Number of pages

7

Pages from-to

611-617

UT code for WoS article

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EID of the result in the Scopus database

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