Production of Polyclonal Antibodies to Potato virus X Using Recombinant Coat Protein
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F10%3A00347697" target="_blank" >RIV/61389030:_____/10:00347697 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Production of Polyclonal Antibodies to Potato virus X Using Recombinant Coat Protein
Original language description
Polyclonal antibodies to recombinant Potato virus X (PVX) coat protein (PVX-CP) were developed and their effectiveness determined in different ELISA protocols. The PVX-CP gene was amplified by reverse transcription-polymerase chain reaction, cloned and expressed in Escherichia coli. For immunization, the CP fractions from bacterial lysate were purified either by simple fractionation or by excision from sodium dodecyl sulphate gels. The PVX-CP was injected into rabbits for antibody production. The PVX-CPantibodies reacted in an indirect plate trapped antigen enzyme-linked immunosorbent assay and immunoblot assay and were useful for the detection of a broad spectrum of isolates of PVX.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/1M06030" target="_blank" >1M06030: Functional genomics and proteomics for crop improvement</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2010
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Phytopathology - Phytopathologische Zeitschrift
ISSN
0931-1785
e-ISSN
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Volume of the periodical
158
Issue of the periodical within the volume
1
Country of publishing house
DE - GERMANY
Number of pages
3
Pages from-to
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UT code for WoS article
000272582000011
EID of the result in the Scopus database
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