Cryotolerance in Norway spruce and its association with growth rates, anatomical features and polyamines of embryogenic cultures
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F10%3A00357035" target="_blank" >RIV/61389030:_____/10:00357035 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Cryotolerance in Norway spruce and its association with growth rates, anatomical features and polyamines of embryogenic cultures
Original language description
The anatomical characteristics and PA content during cryopreservation and regrowth were studied in the ESMs of AFO 541 and C110 cultures, which have comparable ESM anatomy but diverse growth rates, PA content and regeneration abilities after cryopreservation. The endogenous free PAs were maintained at high levels, with spermidine being the predominant PA in the AFO 541, while in C110 the content of putrescine and spermidine was almost identical and rather low, the content of spermidine being approximately one-third that in the ESM of AFO 541. Osmotic pretreatment, using a double application of sorbitol followed by an application of dimethyl sulfoxide (DMSO) resulted in the continual disintegration of polyembryogenic centers and suspensors in both celllines. A continual decrease in the level of PAs was observed during the cell osmotic pretreatment. The cells that retained their viability and regrowth ability after cryopreservation were the meristematic cells inside embryonal heads.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EA - Morphology and cytology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2010
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Tree Physiology
ISSN
0829-318X
e-ISSN
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Volume of the periodical
30
Issue of the periodical within the volume
10
Country of publishing house
GB - UNITED KINGDOM
Number of pages
14
Pages from-to
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UT code for WoS article
000282818100009
EID of the result in the Scopus database
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