Expression of Recombinant Potato leafroll virus Structural and Non-structural Proteins for Antibody Production
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F11%3A00364101" target="_blank" >RIV/61389030:_____/11:00364101 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1111/j.1439-0434.2010.01740.x" target="_blank" >http://dx.doi.org/10.1111/j.1439-0434.2010.01740.x</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/j.1439-0434.2010.01740.x" target="_blank" >10.1111/j.1439-0434.2010.01740.x</a>
Alternative languages
Result language
angličtina
Original language name
Expression of Recombinant Potato leafroll virus Structural and Non-structural Proteins for Antibody Production
Original language description
Vector pMPM-A4 Omega and vectors pQE-30 and pET-45b(+) containing the 6x His-tag sequence were used for expression of Potato leafroll virus (PLRV) structural and non-structural proteins in Escherichia coli. Coat protein (CP) and RNA-dependent RNA polymerase (RdRp)-fragments RdRp(43-616) and RdRp(304-537) were chosen for expression. A high level of CP and RdRp(304-537) was obtained only in an expression system using pET-45b(+) vector and E. coli Rosetta-gami 2(DE3) cells. After purification, the His-tagged PLRV proteins were used for immunization of rabbits.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Phytopathology - Phytopathologische Zeitschrift
ISSN
0931-1785
e-ISSN
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Volume of the periodical
159
Issue of the periodical within the volume
2
Country of publishing house
DE - GERMANY
Number of pages
3
Pages from-to
130-132
UT code for WoS article
000285968400012
EID of the result in the Scopus database
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