Expression of Recombinant Potato leafroll virus Structural and Non-structural Proteins for Antibody Production

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60109807%3A_____%2F11%3A2R000271" target="_blank" >RIV/60109807:_____/11:2R000271 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1111/j.1439-0434.2010.01740.x" target="_blank" >http://dx.doi.org/10.1111/j.1439-0434.2010.01740.x</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/j.1439-0434.2010.01740.x" target="_blank" >10.1111/j.1439-0434.2010.01740.x</a>

Result language

angličtina

Original language name

Expression of Recombinant Potato leafroll virus Structural and Non-structural Proteins for Antibody Production

Original language description

Vector pMPM-A4W and vectors pQE-30 and pET- 45b(+) containing the 6x His-tag sequence were used for expression of Potato leafroll virus (PLRV) structural and non-structural proteins in Escherichia coli. Coat protein (CP) and RNA-dependent RNA polymerase(RdRp)?fragments RdRp43-616 and RdRp304-537 were chosen for expression. A high level of CP and RdRp304-537 was obtained only in an expression system using pET-45b(+) vector and E. coli Rosettagami 2(DE3) cells. After purification, the His-tagged PLRV proteins were used for immunization of rabbits. New Zealand rabbits were immunized either with the recombinant PLRV RdRp304-537 or CP protein,and the antisera obtained were assessed by IPTAELISA using extracts from healthy and PLRV-infected potato plants asantigens. The anti-PLRV CP antibodies were weak and less specific than the commercial PRI antibodies. Antibodies to PLRV non-structural proteins are not available commercially. Although they reacted weakly, our antibodies are the first t

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GF - Diseases, pests, weeds and plant protection

OECD FORD branch

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Project

<a href="/en/project/QH71123" target="_blank" >QH71123: Variability of Potato leafroll luteovirus (PLRV), improved reliability of its detection and utilization of transgenic resistance.</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2011

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Phytopathology

ISSN

1439-0434

e-ISSN

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Volume of the periodical

159

Issue of the periodical within the volume

2

Country of publishing house

DE - GERMANY

Number of pages

3

Pages from-to

130-132

UT code for WoS article

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EID of the result in the Scopus database

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