Chromosome Analysis and Sorting Using Flow Cytometry

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F11%3A00375427" target="_blank" >RIV/61389030:_____/11:00375427 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1007/978-1-61737-957-4_12" target="_blank" >http://dx.doi.org/10.1007/978-1-61737-957-4_12</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/978-1-61737-957-4_12" target="_blank" >10.1007/978-1-61737-957-4_12</a>

Result language
angličtina
Original language name
Chromosome Analysis and Sorting Using Flow Cytometry
Original language description
Chromosome analysis and sorting using flow cytometry (flow cytogenetics) is an attractive tool for fractionating plant genomes to small parts. The reduction of complexity greatly simplifies genetics and genomics in plant species with large genomes. However, as flow cytometry requires liquid suspensions of particles, the lack of suitable protocols for preparation of solutions of intact chromosomes delayed the application of flow cytogenetics in plants. This chapter outlines a high-yielding procedure forpreparation of solutions of intact mitotic chromosomes from root tips of young seedlings, their analysis using flow cytometry and sorting. Seedlings are easy to handle, root meristems are karyological stable, and high degree of metaphase synchrony can beinduced. Chromosomes isolated according to this protocol have well preserved morphology, withstand shearing forces during sorting and their DNA is intact and suitable for a range of applications.
Czech name
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Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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