Flow Cytometric Analysis and Sorting of Plant Chromosomes

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F23%3A00619108" target="_blank" >RIV/61389030:_____/23:00619108 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1007/978-1-0716-3226-0_10" target="_blank" >https://doi.org/10.1007/978-1-0716-3226-0_10</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/978-1-0716-3226-0_10" target="_blank" >10.1007/978-1-0716-3226-0_10</a>

Result language
angličtina
Original language name
Flow Cytometric Analysis and Sorting of Plant Chromosomes
Original language description
Flow cytometry offers a unique way of analyzing and manipulating plant chromosomes. During a rapid movement in a liquid stream, large populations can be classified in a short time according to their fluorescence and light scatter properties. Chromosomes whose optical properties differ from other chromosomes in a karyotype can be purified by flow sorting and used in a range of applications in cytogenetics, molecular biology, genomics, and proteomics. As the samples for flow cytometry must be liquid suspensions of single particles, intact chromosomes must be released from mitotic cells. This protocol describes a procedure for preparation of suspensions of mitotic metaphase chromosomes from meristem root tips and their flow cytometric analysis and sorting for various downstream applications.
Czech name
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Czech description
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Project
<a href="/en/project/EF16_019%2F0000827" target="_blank" >EF16_019/0000827: Plants as a tool for sustainable global development</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Book/collection name
Plant Cytogenetics and Cytogenomics, Methods in Molecular Biology
ISBN
978-1-0716-3225-3
Number of pages of the result
24
Pages from-to
"Roč. 2672 (2023)"
Number of pages of the book
568
Publisher name
Humana
Place of publication
New York
UT code for WoS chapter
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