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Chromosomes in the flow to simplify genome analysis

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F12%3A00381156" target="_blank" >RIV/61389030:_____/12:00381156 - isvavai.cz</a>

  • Result on the web

    <a href="http://dx.doi.org/10.1007/s10142-012-0293-0" target="_blank" >http://dx.doi.org/10.1007/s10142-012-0293-0</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1007/s10142-012-0293-0" target="_blank" >10.1007/s10142-012-0293-0</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Chromosomes in the flow to simplify genome analysis

  • Original language description

    Nuclear genomes of human, animals, and plants are organized into subunits called chromosomes. When isolated into aqueous suspension, mitotic chromosomes can be classified using flow cytometry according to light scatter and fluorescence parameters. Chromosomes of interest can be purified by flow sorting if they can be resolved from other chromosomes in a karyotype. The analysis and sorting are carried out at rates of 10(2)-10(4) chromosomes per second, and for complex genomes such as wheat the flow sorting technology has been ground-breaking in reducing genome complexity for genome sequencing. The high sample rate provides an attractive approach for karyotype analysis (flow karyotyping) and the purification of chromosomes in large numbers. In characterizing the chromosome complement of an organism, the high number that can be studied using flow cytometry allows for a statistically accurate analysis. Chromosome sorting plays a particularly important role in the analysis of nuclear genome

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EB - Genetics and molecular biology

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2012

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    FUNCTIONAL & INTEGRATIVE GENOMICS

  • ISSN

    1438-793X

  • e-ISSN

  • Volume of the periodical

    12

  • Issue of the periodical within the volume

    3

  • Country of publishing house

    DE - GERMANY

  • Number of pages

    20

  • Pages from-to

    397-416

  • UT code for WoS article

    000308238900001

  • EID of the result in the Scopus database