Chromosomes in the flow to simplify genome analysis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F12%3A00381156" target="_blank" >RIV/61389030:_____/12:00381156 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1007/s10142-012-0293-0" target="_blank" >http://dx.doi.org/10.1007/s10142-012-0293-0</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s10142-012-0293-0" target="_blank" >10.1007/s10142-012-0293-0</a>
Alternative languages
Result language
angličtina
Original language name
Chromosomes in the flow to simplify genome analysis
Original language description
Nuclear genomes of human, animals, and plants are organized into subunits called chromosomes. When isolated into aqueous suspension, mitotic chromosomes can be classified using flow cytometry according to light scatter and fluorescence parameters. Chromosomes of interest can be purified by flow sorting if they can be resolved from other chromosomes in a karyotype. The analysis and sorting are carried out at rates of 10(2)-10(4) chromosomes per second, and for complex genomes such as wheat the flow sorting technology has been ground-breaking in reducing genome complexity for genome sequencing. The high sample rate provides an attractive approach for karyotype analysis (flow karyotyping) and the purification of chromosomes in large numbers. In characterizing the chromosome complement of an organism, the high number that can be studied using flow cytometry allows for a statistically accurate analysis. Chromosome sorting plays a particularly important role in the analysis of nuclear genome
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
FUNCTIONAL & INTEGRATIVE GENOMICS
ISSN
1438-793X
e-ISSN
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Volume of the periodical
12
Issue of the periodical within the volume
3
Country of publishing house
DE - GERMANY
Number of pages
20
Pages from-to
397-416
UT code for WoS article
000308238900001
EID of the result in the Scopus database
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