Flow cytometric analysis and sorting of plant chromosomes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F23%3A00579314" target="_blank" >RIV/61389030:_____/23:00579314 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.1007/s13237-023-00450-6" target="_blank" >https://doi.org/10.1007/s13237-023-00450-6</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s13237-023-00450-6" target="_blank" >10.1007/s13237-023-00450-6</a>

Result language

angličtina

Original language name

Flow cytometric analysis and sorting of plant chromosomes

Original language description

Flow cytogenetics is a high-throughput technique that classifies large populations of mitotic chromosomes according to their fluorescence and light scattering as they move in a single file rapidly in a narrow stream of liquid. Flow karyotyping can detect structural and numerical chromosome changes, and chromosomes purified by sorting have been used to discover the three-dimensional organization of DNA, characterize the proteome, and describe the organization of the perichromosomal layer at the nanometre level. However, the most frequent and important use of flow cytogenetics has been the isolation of different chromosomes. Chromosome sorting has made it possible to dissect nuclear genomes into small and defined parts, therefore allowing targeted, simplified, and more economical genomic analyses. The uses of flow-sorted chromosomes for genome complexity reduction and targeted analysis have expanded hand-in-hand with the progress of molecular biology and genomics techniques. These uses include the targeted development of DNA markers, the construction of chromosome-specific DNA libraries, physical mapping, draft genome sequencing, and gene cloning.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10603 - Genetics and heredity (medical genetics to be 3)

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

NUCLEUS

ISSN

0029-568X

e-ISSN

—

Volume of the periodical

66

Issue of the periodical within the volume

3

Country of publishing house

IN - INDIA

Number of pages

15

Pages from-to

355-369

UT code for WoS article

001101463300001

EID of the result in the Scopus database

2-s2.0-85176741896