Assaying nonspecific phospholipase C activity

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F13%3A00423956" target="_blank" >RIV/61389030:_____/13:00423956 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1007/978-1-62703-401-2_18" target="_blank" >http://dx.doi.org/10.1007/978-1-62703-401-2_18</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/978-1-62703-401-2_18" target="_blank" >10.1007/978-1-62703-401-2_18</a>

Result language
angličtina
Original language name
Assaying nonspecific phospholipase C activity
Original language description
Plant nonspecific phospholipase C (NPC) is a recently described enzyme which plays a role in membrane rearrangement during phosphate starvation. It is also involved in responses of plants to brassinolide, abscisic acid (ABA), elicitors, and salt. The NPCactivity is decreased in cells treated with aluminum. In the case of salt stress, the molecular mechanism of NPC action is based on accumulation of diacylglycerol (DAG) by hydrolysis of phospholipids and conversion of DAG, the product of NPC activity, to phosphatidic acid (PA) that participates in ABA signaling pathways. Here we describe a step-by-step protocol, which can be used to determine in situ or in vitro NPC activity. Determination is based on quantification of fluorescently labeled DAG as a product of cleavage of the fluorescently labeled substrate lipid, phosphatidylcholine. High-performance thin-layer chromatography is used for separation of fluorescent DAG. The spot is visualized with a laser scanner and the relative amount
Czech name
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Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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