Mapping nonrecombining regions in barley using multicolor FISH

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F13%3A00424633" target="_blank" >RIV/61389030:_____/13:00424633 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1007/s10577-013-9380-x" target="_blank" >http://dx.doi.org/10.1007/s10577-013-9380-x</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s10577-013-9380-x" target="_blank" >10.1007/s10577-013-9380-x</a>

Result language
angličtina
Original language name
Mapping nonrecombining regions in barley using multicolor FISH
Original language description
Fluorescence in situ hybridization (FISH) is a widely used method to localize DNA sequences on chromosomes. Out of the many uses, FISH facilitates construction of physical maps by ordering contigs of large-insert DNA clones, typically bacterial artificial chromosome (BAC) and establishing their orientation. This is important in genomic regions with low recombination frequency where genetic maps suffer from poor resolution. While BAC clones can be mapped directly by FISH in plants with small genomes, excess of repetitive DNA hampers this application in species with large genomes. Mapping single-copy sequences such as complementary DNA (cDNA) is an attractive alternative. Unfortunately, localization of single-copy sequences shorter than 10 kb remains a challenging task in plants. Here, we present a highly efficient FISH technique that enables unambiguous localization of single copy genes. We demonstrated its utility by mapping 13 out of 15 full-length cDNAs of variable length (2,127-3,40
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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