New insights into the wheat chromosome 4D structure and virtual gene order, revealed by survey pyrosequencing

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F15%3A00446524" target="_blank" >RIV/61389030:_____/15:00446524 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/j.plantsci.2014.12.004" target="_blank" >http://dx.doi.org/10.1016/j.plantsci.2014.12.004</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.plantsci.2014.12.004" target="_blank" >10.1016/j.plantsci.2014.12.004</a>

Result language

angličtina

Original language name

New insights into the wheat chromosome 4D structure and virtual gene order, revealed by survey pyrosequencing

Original language description

Survey sequencing of the bread wheat (Triticum aestivum L.) genome (AABBDD) has been approached through different strategies delivering important information. However, the current wheat sequence knowledge is not complete. The aim of our study is to provide different and complementary set of data for chromosome 4D. A survey sequence was obtained by pyrosequencing of flow-sorted 4DS (7.2x) and 4DL (4.1x) arms. Single ends (SE) and long mate pairs (LMP) reads were assembled into contigs (223 Mb) and scaffolds (65 Mb) that were aligned to Aegilops tauschii draft genome (DD), anchoring 34 Mb to chromosome 4. Scaffolds annotation rendered 822 gene models. A virtual gene order comprising 1973 wheat orthologous gene loci and 381 wheat gene models was built. This order was largely consistent with the scaffold order determined based on a published high density map from the Ae. tauschii chromosome 4, using bin-mapped 4D ESTs as a common reference. The virtual order showed a higher collinearity wi

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Plant Science

ISSN

0168-9452

e-ISSN

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Volume of the periodical

233

Issue of the periodical within the volume

APR 2015

Country of publishing house

IE - IRELAND

Number of pages

13

Pages from-to

200-212

UT code for WoS article

000351247300020

EID of the result in the Scopus database

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