Optical and physical mapping with local finishing enables megabase-scale resolution of agronomically important regions in the wheat genome

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F18%3A00494033" target="_blank" >RIV/61389030:_____/18:00494033 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1186/s13059-018-1475-4" target="_blank" >http://dx.doi.org/10.1186/s13059-018-1475-4</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s13059-018-1475-4" target="_blank" >10.1186/s13059-018-1475-4</a>

Result language

angličtina

Original language name

Optical and physical mapping with local finishing enables megabase-scale resolution of agronomically important regions in the wheat genome

Original language description

Numerous scaffold-level sequences for wheat are now being released and, in this context, we report on a strategy for improving the overall assembly to a level comparable to that of the human genome. nUsing chromosome 7A of wheat as a model, sequence-finished megabase-scale sections of this chromosome were established by combining a new independent assembly using a bacterial artificial chromosome (BAC)-based physical map, BAC pool paired-end sequencing, chromosome-arm-specific mate-pair sequencing and Bionano optical mapping with the International Wheat Genome Sequencing Consortium RefSeq v1.0 sequence and its underlying raw data. The combined assembly results in 18 super-scaffolds across the chromosome. The value of finished genome regions is demonstrated for two approximately 2.5 Mb regions associated with yield and the grain quality phenotype of fructan carbohydrate grain levels. In addition, the 50 Mb centromere region analysis incorporates cytological data highlighting the importance of non-sequence data in the assembly of this complex genome region. nSufficient genome sequence information is shown to now be available for the wheat community to produce sequence-finished releases of each chromosome of the reference genome. The high-level completion identified that an array of seven fructosyl transferase genes underpins grain quality and that yield attributes are affected by five F-box-only-protein-ubiquitin ligase domain and four root-specific lipid transfer domain genes. The completed sequence also includes the centromere.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10603 - Genetics and heredity (medical genetics to be 3)

Project

<a href="/en/project/LO1204" target="_blank" >LO1204: Sustainable development of research in the Centre of the Region Haná</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Genome Biology

ISSN

1474-760X

e-ISSN

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Volume of the periodical

19

Issue of the periodical within the volume

AUG 17

Country of publishing house

GB - UNITED KINGDOM

Number of pages

18

Pages from-to

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UT code for WoS article

000442125100002

EID of the result in the Scopus database

2-s2.0-85051741528