Chromosomal genomics facilitates fine mapping of a Russian wheat aphid resistance gene

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F15%3A00447028" target="_blank" >RIV/61389030:_____/15:00447028 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1007/s00122-015-2512-2" target="_blank" >http://dx.doi.org/10.1007/s00122-015-2512-2</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00122-015-2512-2" target="_blank" >10.1007/s00122-015-2512-2</a>

Result language

angličtina

Original language name

Chromosomal genomics facilitates fine mapping of a Russian wheat aphid resistance gene

Original language description

Making use of wheat chromosomal resources, we developed 11 gene-associated markers for the region of interest, which allowed reducing gene interval and spanning it by four BAC clones. Positional gene cloning and targeted marker development in bread wheatare hampered by high complexity and polyploidy of its nuclear genome. Aiming to clone a Russian wheat aphid resistance gene Dn2401 located on wheat chromosome arm 7DS, we have developed a strategy overcoming problems due to polyploidy and enabling efficient development of gene-associated markers from the region of interest. We employed information gathered by GenomeZipper, a synteny-based tool combining sequence data of rice, Brachypodium, sorghum and barley, and took advantage of a high-density linkage map of Aegilops tauschii. To ensure genome- and locus-specificity of markers, we made use of survey sequence assemblies of isolated wheat chromosomes 7A, 7B and 7D. Despite the low level of polymorphism of the wheat D subgenome, our app

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Theoretical and Applied Genetics

ISSN

0040-5752

e-ISSN

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Volume of the periodical

128

Issue of the periodical within the volume

7

Country of publishing house

US - UNITED STATES

Number of pages

11

Pages from-to

1373-1383

UT code for WoS article

000356148000012

EID of the result in the Scopus database

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