De novo biosynthesis of cytokinins in the biotrophic fungus Claviceps purpurea
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F15%3A00447381" target="_blank" >RIV/61389030:_____/15:00447381 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1111/1462-2920.12838" target="_blank" >http://dx.doi.org/10.1111/1462-2920.12838</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/1462-2920.12838" target="_blank" >10.1111/1462-2920.12838</a>
Alternative languages
Result language
angličtina
Original language name
De novo biosynthesis of cytokinins in the biotrophic fungus Claviceps purpurea
Original language description
Disease symptoms of some phytopathogenic fungi are associated with changes in cytokinin (CK) levels. Here, we show that the CK profile of ergot-infected rye plants is also altered, although no pronounced changes occur in the expression of the host plant's CK biosynthesis genes. Instead, we demonstrate a clearly different mechanism: we report on the first fungal de novoCK biosynthesis genes, prove their functions and constitute a biosynthetic pathway. The ergot fungus Claviceps purpurea produces substantial quantities of CKs in culture and, like plants, expresses enzymes containing the isopentenyltransferase and lonely guy domains necessary for de novo isopentenyladenine production. Uniquely, two of these domains are combined in one bifunctional enzyme,CpIPT-LOG, depicting a novel and potent mechanism for CK production. The fungus also forms trans-zeatin, a reaction catalysed by a CK-specific cytochrome P450 monooxygenase, which is encoded by cpp450 forming a small cluster with cpipt-l
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EI - Biotechnology and bionics
OECD FORD branch
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Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Environmental Microbiology
ISSN
1462-2912
e-ISSN
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Volume of the periodical
17
Issue of the periodical within the volume
8
Country of publishing house
GB - UNITED KINGDOM
Number of pages
17
Pages from-to
2935-2951
UT code for WoS article
000360048800028
EID of the result in the Scopus database
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