De novo biosynthesis of cytokinins in the biotrophic fungus Claviceps purpurea
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F15%3A33156137" target="_blank" >RIV/61989592:15310/15:33156137 - isvavai.cz</a>
Result on the web
<a href="http://onlinelibrary.wiley.com/doi/10.1111/1462-2920.12838/full" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1111/1462-2920.12838/full</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/1462-2920.12838" target="_blank" >10.1111/1462-2920.12838</a>
Alternative languages
Result language
angličtina
Original language name
De novo biosynthesis of cytokinins in the biotrophic fungus Claviceps purpurea
Original language description
Disease symptoms of some phytopathogenic fungi are associated with changes in cytokinin (CK) levels. Here, we show that the CK pro?le of ergot-infected rye plants is also altered, although no pronounced changes occur in the expression of the host plant'sCK biosynthesis genes. Instead, we demonstrate a clearly different mechanism: we report on the ?rst fungal de novo CK biosynthesis genes, prove their functions and constitute a biosynthetic pathway. The ergot fungus Claviceps purpurea produces substantial quantities of CKs in culture and, like plants, expresses enzymes containing the isopentenyltransferase and lonely guy domains necessary for de novo isopentenyladenine production. Uniquely, two of these domains are combined in one bifunctional enzyme,CpIPT-LOG, depicting a novel and potent mechanism for CK production. The fungus also forms trans-zeatin, a reaction catalysed by a CK-speci?c cytochrome P450 monooxygenase, which is encoded by cpp450 forming a small cluster with cpipt-log
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Environmental Microbiology
ISSN
1462-2920
e-ISSN
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Volume of the periodical
17
Issue of the periodical within the volume
8
Country of publishing house
US - UNITED STATES
Number of pages
17
Pages from-to
2935-2951
UT code for WoS article
000360048800028
EID of the result in the Scopus database
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