Arabidopsis non-specific phospholipase C1: Characterization and its involvement in response to heat stress

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F15%3A00451388" target="_blank" >RIV/61389030:_____/15:00451388 - isvavai.cz</a>

Alternative codes found

RIV/60461373:22330/15:43899808

Result on the web

<a href="http://dx.doi.org/10.3389/fpls.2015.00928" target="_blank" >http://dx.doi.org/10.3389/fpls.2015.00928</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3389/fpls.2015.00928" target="_blank" >10.3389/fpls.2015.00928</a>

Result language

angličtina

Original language name

Arabidopsis non-specific phospholipase C1: Characterization and its involvement in response to heat stress

Original language description

The Arabidopsis non-specific phospholipase C1 (NPC) protein family is encoded by the genes NPC1 ? NPC6. It has been shown that NPC4 and NPC5 possess phospholipase C activity; NPC3 has lysophosphatidic acid phosphatase activity. NPC3, 4 and 5 play roles in the responses to hormones and abiotic stresses. NPC1, 2 and 6 has not been studied functionally yet. We found that Arabidopsis NPC1 expressed in Escherichia coli possesses phospholipase C activity in vitro. This protein was able to hydrolyse phosphatidylcholine to diacylglycerol. NPC1-green fluorescent protein was localized to secretory pathway compartments in Arabidopsis roots. In the knock out T-DNA insertion line NPC1 (npc1) basal thermotolerance was impaired compared with wild-type (WT); npc1 exhibited significant decreases in survival rate and chlorophyll content at the seventh day after heat stress (HS). Conversely, plants overexpressing NPC1 (NPC1-OE) were more resistant to HS compared with WT. These findings suggest that NPC1

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

ED - Physiology

OECD FORD branch

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Project

<a href="/en/project/GAP501%2F12%2F1942" target="_blank" >GAP501/12/1942: Nonspecific phospholipase C: Molecular, cellular and functional characterisation of novel plant enzyme</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Frontiers in Plant Science

ISSN

1664-462X

e-ISSN

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Volume of the periodical

6

Issue of the periodical within the volume

NOV 4

Country of publishing house

CH - SWITZERLAND

Number of pages

13

Pages from-to

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UT code for WoS article

000364581700001

EID of the result in the Scopus database

2-s2.0-84947424867