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An efficient approach to BAC based assembly of complex genomes

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F16%3A00456187" target="_blank" >RIV/61389030:_____/16:00456187 - isvavai.cz</a>

  • Result on the web

    <a href="http://dx.doi.org/10.1186/s13007-016-0107-9" target="_blank" >http://dx.doi.org/10.1186/s13007-016-0107-9</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1186/s13007-016-0107-9" target="_blank" >10.1186/s13007-016-0107-9</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    An efficient approach to BAC based assembly of complex genomes

  • Original language description

    Background: There has been an exponential growth in the number of genome sequencing projects since the introduction of next generation DNA sequencing technologies. Genome projects have increasingly involved assembly of whole genome data which produces inferior assemblies compared to traditional Sanger sequencing of genomic fragments cloned into bacterial artificial chromosomes (BACs). While whole genome shotgun sequencing using next generation sequencing (NGS) is relatively fast and inexpensive, this method is extremely challenging for highly complex genomes, where polyploidy or high repeat content confounds accurate assembly, or where a highly accurate 'gold' reference is required. Several attempts have been made to improve genome sequencing approaches by incorporating NGS methods, to variable success. nnResults: We present the application of a novel BAC sequencing approach which combines indexed pools of BACs, Illumina paired read sequencing, a sequence assembler specifically desig

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EB - Genetics and molecular biology

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2016

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Plant Methods

  • ISSN

    1746-4811

  • e-ISSN

  • Volume of the periodical

    12

  • Issue of the periodical within the volume

    JAN 20

  • Country of publishing house

    GB - UNITED KINGDOM

  • Number of pages

    9

  • Pages from-to

  • UT code for WoS article

    000368412000001

  • EID of the result in the Scopus database