An efficient approach to BAC based assembly of complex genomes
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F16%3A00456187" target="_blank" >RIV/61389030:_____/16:00456187 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1186/s13007-016-0107-9" target="_blank" >http://dx.doi.org/10.1186/s13007-016-0107-9</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1186/s13007-016-0107-9" target="_blank" >10.1186/s13007-016-0107-9</a>
Alternative languages
Result language
angličtina
Original language name
An efficient approach to BAC based assembly of complex genomes
Original language description
Background: There has been an exponential growth in the number of genome sequencing projects since the introduction of next generation DNA sequencing technologies. Genome projects have increasingly involved assembly of whole genome data which produces inferior assemblies compared to traditional Sanger sequencing of genomic fragments cloned into bacterial artificial chromosomes (BACs). While whole genome shotgun sequencing using next generation sequencing (NGS) is relatively fast and inexpensive, this method is extremely challenging for highly complex genomes, where polyploidy or high repeat content confounds accurate assembly, or where a highly accurate 'gold' reference is required. Several attempts have been made to improve genome sequencing approaches by incorporating NGS methods, to variable success. nnResults: We present the application of a novel BAC sequencing approach which combines indexed pools of BACs, Illumina paired read sequencing, a sequence assembler specifically desig
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Plant Methods
ISSN
1746-4811
e-ISSN
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Volume of the periodical
12
Issue of the periodical within the volume
JAN 20
Country of publishing house
GB - UNITED KINGDOM
Number of pages
9
Pages from-to
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UT code for WoS article
000368412000001
EID of the result in the Scopus database
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