Development and optimization of ultra-high performance supercritical fluid chromatography mass spectrometry method for high-throughput determination of tocopherols and tocotrienols in human serum
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F16%3A00463765" target="_blank" >RIV/61389030:_____/16:00463765 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11160/16:10328691 RIV/61989592:15310/16:33161770 RIV/60193697:_____/16:N0000072
Result on the web
<a href="http://dx.doi.org/10.1016/j.aca.2016.06.008" target="_blank" >http://dx.doi.org/10.1016/j.aca.2016.06.008</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.aca.2016.06.008" target="_blank" >10.1016/j.aca.2016.06.008</a>
Alternative languages
Result language
angličtina
Original language name
Development and optimization of ultra-high performance supercritical fluid chromatography mass spectrometry method for high-throughput determination of tocopherols and tocotrienols in human serum
Original language description
The goal of this study was to develop an effective supercritical fluid chromatography method using single quadrupole MS for analysis of all isomeric forms of vitamin E. Finally, two fast and effective methods, the high resolution one and the high speed one, for the determination of 8 vitamin E isomers in human serum were developed.nRapid high-throughput liquid-liquid extraction was selected as a sample preparation step. Sample pretreatment of 100 mu L human serum was consisted of protein precipitation with 200 mL ethanol and liquid-liquid extraction by 400 mu L hexane/dichloromethane (80/20, v/v). The separation was performed on BEH 2-EP (3.0 x 100 mu m, 1.7 mu m) stationary phase, using isocratic elution with carbon dioxide and 10 mu M ammonium formate in methanol in the ratio 98: 2 for high resolution method with run time 4.5 min and in the ratio 95: 5 for high speed method, where the run time was 2.5 min. The method development included optimization of key parameters: the choice of the suitable stationary phase and the composition of mobile phase, where an influence of various modifiers, their ratio and additives were tested, and optimization of fine tunning parameters including BPR pressure, flow-rate and column temperature. Quantification of all isomeric forms was performed using SIM (single ion monitoring) experiments in ESI positive ion mode. Both high speed and high resolution chromatographic methods were validated in terms of precision, accuracy, range, linearity, LOD, LOQ and matrix effects using the same LLE procedure. The high resolution method provided more sensitive results (LOD: 0.017-0.083 mu g mL(-1)) and better linearity (r(2) > 0.9930) than the high speed one (LOD: 0.083-0.25 mg mL(-1), r(2) > 0.9877) at the cost of double time of analysis.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/LO1204" target="_blank" >LO1204: Sustainable development of research in the Centre of the Region Haná</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Analytica Chimica Acta
ISSN
0003-2670
e-ISSN
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Volume of the periodical
934
Issue of the periodical within the volume
AUG 31
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
14
Pages from-to
252-265
UT code for WoS article
000381928900030
EID of the result in the Scopus database
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