Flow Sorting Plant Chromosomes

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F16%3A00464630" target="_blank" >RIV/61389030:_____/16:00464630 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1007/978-1-4939-3622-9_10" target="_blank" >http://dx.doi.org/10.1007/978-1-4939-3622-9_10</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/978-1-4939-3622-9_10" target="_blank" >10.1007/978-1-4939-3622-9_10</a>

Result language
angličtina
Original language name
Flow Sorting Plant Chromosomes
Original language description
Nuclear genomes of many important plant species are tremendously complicated to map and sequence. The ability to isolate single chromosomes, which represent small units of nuclear genome, is priceless in many areas of plant research including cytogenetics, genomics, and proteomics. Flow cytometry is the only technique which can provide large quantities of pure chromosome fractions suitable for downstream applications including physical mapping, preparation of chromosome-specific BAC libraries, sequencing, and optical mapping. Here, we describe step-by-step procedure of preparation of liquid suspensions of intact mitotic metaphase chromosomes and their flow cytometric analysis and sorting.
Czech name
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Czech description
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Project
<a href="/en/project/LO1204" target="_blank" >LO1204: Sustainable development of research in the Centre of the Region Haná</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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