A High Resolution Radiation Hybrid Map of Wheat Chromosome 4A
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F17%3A00473676" target="_blank" >RIV/61389030:_____/17:00473676 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.3389/fpls.2016.02063" target="_blank" >http://dx.doi.org/10.3389/fpls.2016.02063</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3389/fpls.2016.02063" target="_blank" >10.3389/fpls.2016.02063</a>
Alternative languages
Result language
angličtina
Original language name
A High Resolution Radiation Hybrid Map of Wheat Chromosome 4A
Original language description
Bread wheat has a large and complex allohexaploid genome with low recombination level at chromosome centromeric and peri-centromeric regions. This significantly hampers ordering of markers, contigs of physical maps and sequence scaffolds and impedes obtaining of high-quality reference genome sequence. Here we report on the construction of high-density and high-resolution radiation hybrid (RH) map of chromosome 4A supported by high-density chromosome deletion map. A total of 119 endosperm-based RH lines of two RH panels and 15 chromosome deletion bin lines were genotyped with 90K iSelect single nucleotide polymorphism (SNP) array. A total of 2316 and 2695 markers were successfully mapped to the 4A RH and deletion maps, respectively. The chromosome deletion map was ordered in 19 bins and allowed precise identification of centromeric region and verification of the RH panel reliability. The 4A-specific RH map comprises 1080 mapping bins and spans 6550.9 cR with a resolution of 0.13 Mb/cR. Significantly higher mapping resolution in the centromeric region was observed as compared to recombination maps. Relatively even distribution of deletion frequency along the chromosome in the RH panel was observed and putative functional centromere was delimited within a region characterized by two SNP markers.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10611 - Plant sciences, botany
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Frontiers in Plant Science
ISSN
1664-462X
e-ISSN
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Volume of the periodical
7
Issue of the periodical within the volume
JAN 10
Country of publishing house
CH - SWITZERLAND
Number of pages
14
Pages from-to
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UT code for WoS article
000391502500001
EID of the result in the Scopus database
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