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A High Resolution Radiation Hybrid Map of Wheat Chromosome 4A

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F17%3A00473676" target="_blank" >RIV/61389030:_____/17:00473676 - isvavai.cz</a>

  • Result on the web

    <a href="http://dx.doi.org/10.3389/fpls.2016.02063" target="_blank" >http://dx.doi.org/10.3389/fpls.2016.02063</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.3389/fpls.2016.02063" target="_blank" >10.3389/fpls.2016.02063</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    A High Resolution Radiation Hybrid Map of Wheat Chromosome 4A

  • Original language description

    Bread wheat has a large and complex allohexaploid genome with low recombination level at chromosome centromeric and peri-centromeric regions. This significantly hampers ordering of markers, contigs of physical maps and sequence scaffolds and impedes obtaining of high-quality reference genome sequence. Here we report on the construction of high-density and high-resolution radiation hybrid (RH) map of chromosome 4A supported by high-density chromosome deletion map. A total of 119 endosperm-based RH lines of two RH panels and 15 chromosome deletion bin lines were genotyped with 90K iSelect single nucleotide polymorphism (SNP) array. A total of 2316 and 2695 markers were successfully mapped to the 4A RH and deletion maps, respectively. The chromosome deletion map was ordered in 19 bins and allowed precise identification of centromeric region and verification of the RH panel reliability. The 4A-specific RH map comprises 1080 mapping bins and spans 6550.9 cR with a resolution of 0.13 Mb/cR. Significantly higher mapping resolution in the centromeric region was observed as compared to recombination maps. Relatively even distribution of deletion frequency along the chromosome in the RH panel was observed and putative functional centromere was delimited within a region characterized by two SNP markers.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10611 - Plant sciences, botany

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2017

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Frontiers in Plant Science

  • ISSN

    1664-462X

  • e-ISSN

  • Volume of the periodical

    7

  • Issue of the periodical within the volume

    JAN 10

  • Country of publishing house

    CH - SWITZERLAND

  • Number of pages

    14

  • Pages from-to

  • UT code for WoS article

    000391502500001

  • EID of the result in the Scopus database