Micro-SPE in pipette tips as a tool for analysis of small-molecule drugs in serum
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F17%3A00476523" target="_blank" >RIV/61389030:_____/17:00476523 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11160/17:10361156 RIV/61989592:15310/17:73584793
Result on the web
<a href="http://dx.doi.org/10.4155/bio-2017-0033" target="_blank" >http://dx.doi.org/10.4155/bio-2017-0033</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.4155/bio-2017-0033" target="_blank" >10.4155/bio-2017-0033</a>
Alternative languages
Result language
angličtina
Original language name
Micro-SPE in pipette tips as a tool for analysis of small-molecule drugs in serum
Original language description
Micro-SPE in pipette tips (μ-SPE-PT) with particle sorbent has never been used in small-molecule drug analysis. Methodology & results: μ-SPE-PT was used for the extraction of statins from biological materials followed by UHPLC-MS/MS. The commercial and homemade μ-SPE-PT tips filled with particle sorbent were compared. While the homemade tips enabled direct serum sample loading into the sorbent, protein precipitation (PP) had to be implemented before μ-SPE-PT procedure using commercial tips. Three μ-SPE-PT methods were developed and validated: method A: μ-SPE-PT with homemade tips, method B: PP + μ-SPE-PT with homemade tips, and method C: PP + μ-SPE-PT with commercial tips. Method A enabled a simple high-throughput approach (48 samples in 90 min) compared with methods B and C that required three-times longer time. However, PP increased the recoveries of protein-bound analytes and extracts purity in methods B and C. The matrix effects without internal standards correction for method C were significantly higher than those for the methods A and B. Conclusion: Compared with commercial tips, homemade tips filled with particles were found to be more suitable for drug analysis. Commercial tips tested in this study were found challenging but the conditions under which they could be applicable were also defined.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
<a href="/en/project/LO1204" target="_blank" >LO1204: Sustainable development of research in the Centre of the Region Haná</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Bioanalysis
ISSN
1757-6180
e-ISSN
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Volume of the periodical
9
Issue of the periodical within the volume
11
Country of publishing house
GB - UNITED KINGDOM
Number of pages
14
Pages from-to
887-901
UT code for WoS article
000405109200006
EID of the result in the Scopus database
2-s2.0-85021438825