Phosphoprotein enrichment from tobacco mature pollen crude protein extract
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F17%3A00485921" target="_blank" >RIV/61389030:_____/17:00485921 - isvavai.cz</a>
Result on the web
<a href="http://gateway.isiknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=Alerting&SrcApp=Alerting&DestApp=MEDLINE&DestLinkType=FullRecord&UT=28936665" target="_blank" >http://gateway.isiknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=Alerting&SrcApp=Alerting&DestApp=MEDLINE&DestLinkType=FullRecord&UT=28936665</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/978-1-4939-7286-9_21" target="_blank" >10.1007/978-1-4939-7286-9_21</a>
Alternative languages
Result language
angličtina
Original language name
Phosphoprotein enrichment from tobacco mature pollen crude protein extract
Original language description
Protein phosphorylation was repeatedly shown to be the most dynamic post-translational modification mediated by a huge orchestra of protein kinases and phosphatases. Upon landing on a stigma, pollen grain dehydration and activation are accompanied by changes in protein phosphorylation together with the translation activation of stored mRNAs. To enable studies of the total phosphoproteome, it is usually necessary to apply various enrichment techniques. In this chapter, one of these protocols that worked previously well on tobacco mature pollen is presented in more detail. The method comprises of three basic steps: (1) picking flowers from the flowering tobacco plants (Nicotiana tabacum cv. Samsun), and collection of the shed pollen grains, (2) extraction of total proteins by TCA/acetone, (3) phosphoprotein enrichment by MOAC with aluminum hydroxide matrix. Taken together this protocol describes how to isolate phosphoproteins out of tobacco mature pollen.
Czech name
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Czech description
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Classification
Type
C - Chapter in a specialist book
CEP classification
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OECD FORD branch
10611 - Plant sciences, botany
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Book/collection name
Plant Germline Development
ISBN
978-1-4939-7285-2
Number of pages of the result
10
Pages from-to
"Roč. 1669 (2017)"
Number of pages of the book
422
Publisher name
Humana Press
Place of publication
New York
UT code for WoS chapter
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