Revealing phosphoproteins playing role in tobacco pollen activated in vitro

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F12%3A00384621" target="_blank" >RIV/61389030:_____/12:00384621 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1002/pmic.201100318" target="_blank" >http://dx.doi.org/10.1002/pmic.201100318</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/pmic.201100318" target="_blank" >10.1002/pmic.201100318</a>

Result language

angličtina

Original language name

Revealing phosphoproteins playing role in tobacco pollen activated in vitro

Original language description

The transition between the quiescent mature and the metabolically active germinating pollen grain most probably involves changes in protein phosphorylation status, since phosphorylation has been implicated in the regulation of many cellular processes. Given that, only a minor proportion of cellular proteins are phosphorylated at any one time, and that phosphorylated and nonphosphorylated forms of many proteins can co-exist within a cell, the identification of phosphoproteins requires some prior enrichment from a crude protein extract. Here, we have used metal oxide/hydroxide affinity chromatography (MOAC) based on an aluminum hydroxide matrix for this purpose, and have generated a population of phosphoprotein candidates from both mature and in vitro activated tobacco pollen grains. Both electrophoretic and nonelectrophoretic methods, allied to MS, were applied to these extracts to identify a set of 139 phosphoprotein candidates. In vitro phosphorylation was also used to validate the sp

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

ED - Physiology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Proteomics

ISSN

1615-9853

e-ISSN

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Volume of the periodical

12

Issue of the periodical within the volume

21

Country of publishing house

DE - GERMANY

Number of pages

22

Pages from-to

3229-3250

UT code for WoS article

000310564100012

EID of the result in the Scopus database

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