Specificity of MOAC enrichment applied for mature pollen phosphoproteomics studies
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F12%3A00387762" target="_blank" >RIV/61389030:_____/12:00387762 - isvavai.cz</a>
Result on the web
<a href="http://home.ueb.cas.cz/publikace/2012_Fila_IEEE.pdf" target="_blank" >http://home.ueb.cas.cz/publikace/2012_Fila_IEEE.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1109/iCBEB.2012.362" target="_blank" >10.1109/iCBEB.2012.362</a>
Alternative languages
Result language
angličtina
Original language name
Specificity of MOAC enrichment applied for mature pollen phosphoproteomics studies
Original language description
Androgenesis represents one of the most important and fast-developing biotechnological approaches for the production of haploid and di-haploid crop plants with great potential for plant breeding. Tobacco serves as a suitable model plant with available protocols for androgenesis. However, it is necessary to understand the molecular regulatory mechanisms underlying this process. Phosphorylation is the most dynamic posttranslational modification. In a time, the cell contains only few percent of phosphoproteins that can furthermore be accompanied by their native forms. Thus, the enrichment techniques have to be applied in order to concentrate the phosphorylated forms. The aim of our studies was to compare the phosphoproteins extracted by TCA/acetone out ofmature pollen and 30-min-activated pollen. The crude protein extracts were enriched by the metaloxide/hydroxide affinity chromatography (MOAC) with aluminium hydroxide matrix. The efficiency of the enrichment was verified by 1D SDS-PAGE
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
ED - Physiology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
Proceeding of International Conference on Biomedical Engineering and Biotechnology
ISBN
978-0-7695-4706-0
ISSN
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e-ISSN
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Number of pages
4
Pages from-to
523-526
Publisher name
IEEE Computer Society
Place of publication
Washington, D.C
Event location
Macau
Event date
May 28, 2012
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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