Silver ions increase plasma membrane permeability through modulation of intracellular calcium levels in tobacco BY-2 cells

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F18%3A00489217" target="_blank" >RIV/61389030:_____/18:00489217 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11310/18:10379372

Result on the web

<a href="http://dx.doi.org/10.1007/s00299-018-2269-6" target="_blank" >http://dx.doi.org/10.1007/s00299-018-2269-6</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00299-018-2269-6" target="_blank" >10.1007/s00299-018-2269-6</a>

Result language

angličtina

Original language name

Silver ions increase plasma membrane permeability through modulation of intracellular calcium levels in tobacco BY-2 cells

Original language description

Silver ions increase plasma membrane permeability for water and small organic compounds through their stimulatory effect on plasma membrane calcium channels, with subsequent modulation of intracellular calcium levels and ion homeostasis. Abstract: The action of silver ions at the plant plasma membrane is largely connected with the inhibition of ethylene signalling thanks to the ability of silver ion to replace the copper cofactor in the ethylene receptor. A link coupling the action of silver ions and cellular auxin efflux has been suggested earlier by their possible direct interaction with auxin efflux carriers or by influencing plasma membrane permeability. Using tobacco BY-2 cells, we demonstrate here that besides a dramatic increase of efflux of synthetic auxins 2,4-dichlorophenoxyacetic acid (2,4-D) and 1-naphthalene acetic acid (NAA), treatment with AgNO 3 resulted in enhanced efflux of the cytokinin trans-zeatin (tZ) as well as the auxin structural analogues tryptophan (Trp) and benzoic acid (BA). The application of AgNO 3 was accompanied by gradual water loss and plasmolysis. The observed effects were dependent on the availability of extracellular calcium ions (Ca 2+ ) as shown by comparison of transport assays in Ca 2+rich and Ca 2+free buffers and upon treatment with inhibitors of plasma membrane Ca 2+permeable channels Al 3+ and ruthenium red, both abolishing the effect of AgNO 3 . Confocal microscopy of Ca 2+sensitive fluorescence indicator Fluo-4FF, acetoxymethyl (AM) ester suggested that the extracellular Ca 2+ availability is necessary to trigger the response to silver ions and that the intracellular Ca 2+ pool alone is not sufficient for this effect. Altogether, our data suggest that in plant cells the effects of silver ions originate from the primal modification of the internal calcium levels, possibly by their interaction with Ca 2+permeable channels at the plasma membrane.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10601 - Cell biology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Plant Cell Reports

ISSN

0721-7714

e-ISSN

—

Volume of the periodical

37

Issue of the periodical within the volume

5

Country of publishing house

DE - GERMANY

Number of pages

10

Pages from-to

809-818

UT code for WoS article

000429927300009

EID of the result in the Scopus database

2-s2.0-85044975188